Generator

Part:BBa_K223057:Design

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


Rear Flanking Sequence (HlyB and HlyD)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3673
    Illegal PstI site found at 1187
    Illegal PstI site found at 3581
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 3674
    Illegal PstI site found at 1187
    Illegal PstI site found at 3581
    Illegal PstI site found at 3688
    Illegal NotI site found at 7
    Illegal NotI site found at 3681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3674
    Illegal PstI site found at 1187
    Illegal PstI site found at 3581
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 3674
    Illegal PstI site found at 1187
    Illegal PstI site found at 3581
    Illegal PstI site found at 3688
    Illegal AgeI site found at 1359
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3199


Design Notes

Stanford iGEM Team 2009 altered sequences for internal restriction sites


Source

Compiled from several different sources:

1. GenBank E. coli str. CFT073 

   http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3422041&to=3424164&report=fasta

2. GenBank E. coli str. CFT073 

    http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3424183&to=3425619&report=fasta


References